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Amylase activity of a
yellow pigmented bacterium isolated from cassava waste
Saliu, Bolanle Kudirat
Department of Microbiology, University of Ilorin,
Ilorin, Nigeria.
E-mail:
kudisaliu1964@yahoo.com or
saliu.bk@unilorin.edu.nig.
Tel.: +2348023093294.
Accepted
24 August, 2009 |
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This study
investigated the amylase activity of a yellow pigmented
bacterium isolated from cassava wastes obtained from a
dumpsite near a gari processing factory in Ibadan, Nigeria.
Isolate was grown in nutrient broth containing 1% starch and
then centrifuged at 5,000 rpm. Amylase activity was assayed
using the DNSA method for the detection of reducing sugar.
Best enzyme activity was recorded at 30ºC and pH 7.5. The
amylase was found to be stable after exposure to
temperatures between 20 and 40ºC and pH 6.0 to 9.0, outside
which there was a decline in activity. The ability of the
isolate to produce amylase explains its colonization of a
cassava waste dumpsite. Conditions of activity and stability
also agree with the growth condition of the isolate.
Key
words:
Cassava waste, yellow pigmented bacterium, amylase. |
