Full Length Research Paper

In vitro assessment of cytotoxicity of giomer on human gingival fibroblasts

Reza Pourabbas¹, Safar Farajnia²*, Soodabeh Kimyai³, Leila Mohammadnejad³, Antony Johnson⁴ and Touraj Nejatian⁴

¹Drug Applied Research Center, Tabriz University, Medical Sciences, Tabriz, Iran.

²Biotechnology Research Center, Tabriz University, Medical Sciences, Tabriz, Iran.

³Faculty of Dentistry, Department of Operative Dentistry, Tabriz University, Medical Sciences, Tabriz, Iran.

⁴Department of Adult Dental Care, School of Clinical Dentistry, University of Sheffield, Sheffield, UK.

*Corresponding author. E-mail: farajnias@tbzmed.ac.ir. Tel: 0098411-3363234.

Accepted 15 May, 2009

   Abstract

Root coverage on restored root surfaces has been considered as a challenging issue. The evaluation of cytotoxic effects of restorative materials is a fundamental requirement for sustaining the cell attachment and the clinical success of root coverage. The aim of the present study was to compare the human gingival fibroblast cytotoxicity of the recently introduced giomer composite (GC) with resin ionomer (RI) restorative material. Discs (6×2 mm) of GC and RI restorative materials were prepared using sterile Teflon mold. Extracts from the materials were incubated to cell culture medium for 24, 48 and 72 h. Human gingival fibroblasts (HGF) were exposed to the extracts of the materials while the un-incubated media served as the control group. The cytotoxicity of the materials were evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In order to compare the mean values of the measured parameters a Kruskal-Walis test was carried out. MTT assay indicated that human gingival fibroblasts proliferated well in the presence of GC extract. The proliferation rate was higher in cells incubated with GC compared to RI extracts but the differences were not statistically significant (p= 0.09). This in vitro study indicated that GC is a non-toxic material for HGF. However, further studies are needed to assess the other biologic and clinical behavior of this material prior to it being considered as a potentially suitable restorative material to restore the carious root lesions candidated to root coverage procedures.

Key words: Root coverage material, giomer, ionomer, cell cytotoxicity.