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Evaluation of
interspecific DNA variability in poplars using AFLP and SSR
markers
Orlović Saša1*,
Galović Vladislava1, Zorić Miroslav2,
Kovačević Branislav1, Pilipović Andrej1 and
Zoran Galic1
1University
of Novi Sad, Institute of Lowland Forestry and Environment,
Antona Čehova 13, 21000 Novi Sad, Serbia.
2Institute
of Field Crop Science, Faculty of Agriculture, University of
Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia.
*Corresponding author. E -mail:
sasao@uns.ac.rs. Tel:
+381 21 540 383.
Fax: +381
21 540 385.
Accepted
10 September, 2009 |
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The
objective of this paper was to examine interspecific DNA
variation in poplars using AFLP and SSR markers. The AFLP
and SSR markers polymorphism and its power of discrimination
were determined within 13 genotypes of different genetic
background (clones, cultivars, hybrids) of two sections (Aigeiros
and Tacamahaca) of genus Populus. Twelve sets
of PTR and four sets of WPMS microsatellite primers as well
as 6 AFLP primer combinations were used in this study. SSR
and AFLP markers revealed high power of discrimination, 0.81
and 0.92 respectively. Results obtained using SSR data
showed a clear separation of three major clusters, while
four major clusters were obtained using AFLP data. Both
markers crearly separated two distinct clusters, one
included Populus nigra and the other Populus
deltoides genotypes. According to both markers,
different genetic background were revealed between two
clones of Populus trichocarpa. When evaluating
polymorphisms within genotypes of interest, microsatellite
and AFLP DNA markers proved to be a useful tool for
distinguishing genetic background of tree clones, cultivars
and hybrid genotypes thus grouping them according to their
genetic dissimilarity. The results presented in this study
could be of significant interest in poplar breeding programs
and could also be used as a valuable annex in new bred
clones registration process.
Key
words:
Poplar, AFLP and SSR markers, genotyping, polymorphisms. |
