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Molecular characterization
and phylogenetic relationships among and within species of
Phalaenopsis (Epidendroideae: Orchidaceae) based on
RAPD analysis
A. Niknejad1*,
M. A. Kadir1, S. B. Kadzimin2,
N. A. P. Abdullah2 and
K. Sorkheh3
1Department
of Agriculture Technology, Faculty of Agriculture,
University Putra, Malaysia.
2Department
of Crop Science, Faculty of Agriculture, University Putra,
Malaysia.
3Department
of Agronomy and Plant Breeding, Faculty of Agriculture,
Shahr-e-Kord University, Iran.
*Corresponding author.
E-mail:
niknejad.azadeh@gmail.com. Tel: 0060172071690.
Accepted 31 August, 2009 |
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Random
amplified polymorphic DNA (RAPD) analysis for 20 species of
Phalaenopsis was conducted to determine their genetic
distances and relationships. Among 20 different primers used
for RAPD analysis, 10 primers showed polymorphism, and
according to the primer type, 26 to 54 DNA fragments were
amplified. A total of 414 polymorphic fragments were
generated by 10 primers and used for correlation group
analysis. The highest value of Similarity index was 0.28
between Ph. violacea malaysia and Ph. violacea
witte. The dendrogram resulting from UPGMA (Unweighted
Pair Group Method using Arithmetic average) hierarchical
cluster analysis separated the original species into three
groups: The first group had five species of Ph. violacea
blue, Ph. belina, Ph. violacea malaysia,
Ph. violacea witte, and Ph. gigantea; the
second group included Ph. lamelligera, Ph. amabilis, Ph.
parishii, Ph. labbi nepal, Ph. speciosa, Ph. lobbi yellow,
Ph. venosa, Ph. hieroglyphica, and Ph. maculata;
the third group consisted of Ph. minho princess, Ph.
leopard prince, Ph. mannii, Ph. modesta, Ph. cornucervi
and Ph. pantherina. RAPD markers can thus be
successfully applied in this economically important group of
orchids for the study of molecular characterization and
relationships. The data acquired from this study could be
used for identification and classification of other orchid
genera and oriental Phalaenopsis.
Key
words:
Phalaenopsis, Random Amplified Polymorphic DNA (RAPD),
classification, molecular characterization. |
