|
Ascorbic acid effects on
in vitro maturation of mouse oocyte with or without
cumulus cell
Behnaz Nadri*, Saeed
Zeinoaldini and Hamid Kohram
Animal Science group, Faculty of Agronomy and Animal
Science, University of Tehran, Karaj, Iran.
*Corresponding author.
E -mail:
behnaznadri2000@yahoo.com.
Tel.:
0098-21-44230529. Fax: 0098-21-44230529.
Accepted
24 August, 2009 |
|
Ascorbic
acid has long been associated with fertility. This study was
designed to determine the effects of ascorbic acid on in
vitro maturation of mouse oocyte with or without cumulus
cells. In this study, 508 denuded oocytes (DOs) and 527
cumulus–oocyte complexes (COCs) from mice stimulated with
pregnant mare’s serum gonadotrophin (PMSG) were incubated
for 24 h in medium containing 0, 80, 250 and 750 μM/ml of
ascorbic acid prior to in vitro maturation.
Maturation rate was compared. A significant decrease in the
maturation rate was observed only when the DOs and COCs were
exposed to 750 μM/ml of ascorbic acid (P < 0.05). The
maturation rate in COCs was significantly higher than DOs in
all groups (P < 0.05). These results indicate that exposure
ascorbic acid promotes the development of mouse DOs and COCs
from germinal vesicle breakdown (GVBD) to metaphase II (MII)
and prevents cumulus cell degeneration at certain levels,
especially 250 μM/ml of ascorbic acid (P < 0.05). However,
further studies on the potential effects of different
concentrations of ascorbic acid on oocyte maturation are
needed.
Key
words:
Ascorbic
acid, cumulus cell, in vitro maturation, mice. |
