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Expression analysis of
four flower-specific promoters of Brassica spp. in
the heterogeneous host
tobacco
An-Qi Geng, Zhan-Jun Zhao,
Xuan-Li Nie and Xing-Guo Xiao*
State
Key Laboratory of Plant Physiology and Biochemistry, College
of Biological Sciences, China Agricultural University,
Beijing, 100094 China.
*Corresponding author.
E-mail: xiaoxg@cau.edu.cn
or
xiaoxg06@gmail.com.
Tel.:
+86-10-62731324.
Fax: +86-10-62733491.
Accepted
31 August, 2009 |
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The
5’-flanking region of ca. 1200 bp upstream of the
translation start site (TSS) of a putative cell wall protein
gene was cloned from Brassica campestris, B.
chinensis, B. napus and B. oleracea, and
transferred to tobacco via Agrobacterium-mediation
after fused to promoter-less beta-glucuronidase (GUS)
reporter gene. Histochemical GUS staining and fluorometric
quantification of the transgenic tobacco showed that all
four promoters conferred GUS expression in petal, anther,
pollen and stigma of the
flower, not in any vegetative organs or tissues of the
plants. A series of 5’-end deletion of the promoter from
B. napus disclosed that the region -104 to -17 relative
to TSS was sufficient to confer flower-specific expression,
and the region -181 to -161 played a key role in maintaining
strong driving power of the promoter. Besides, several
enhancer and suppressor regions were also identified in the
promoter.
Key
words:
Flower-specific promoter, floral-specific promoter,
Brassica campestris, Brassica chinensis,
Brassica napus, Brassica oleracea, minimal
promoter, enhancer, suppressor, coordinated expression. |
