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Effects of NaCl treatment
on the antioxidant enzymes of oilseed rape (Brassica
napus
L.) seedlings
Dai Qi-lin1,
Chen Chen3, Feng Bin2, Liu Ting-ting2,
Tian Xia2, Gong Yuan-ya2, Sun Ying-kun2,
Wang Jin1, 2 and Du Shi-zhang1*
1Plant
Genomics Research Center, Mianyang Normal College, Mianyang
621000, Sichuan, China.
2Key
Laboratory for Nuclear Waste Treatment and Environmental
Safety (SWUST), Commission of Science, Technology and
Industry for National Defence, Southwest University of
Science and Technology, Mianyang 621000, China.
3School
of Pharmacy, Fudan University, Shanghai 200030, China.
*Corresponding author.
E-mail:
wjdsz@vip.sina.com.cn.
Accepted
10 September, 2009 |
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The effects
of NaCl treatment on the activity of antioxidant enzymes in
leaves of oilseed rape seedlings (Brassica
napus L.) were studied. The results showed that the relative
water content from leaves of oilseed rape seedlings was
gradually decreased and the electronic conductivity was
increased during 0 - 24 h under 200 mmol.l-1 NaCl
treatments. The activity of peroxidase (POD), superoxide
dismutase (SOD) and catalase (CAT) was gradually increased
during 0 - 24 h under 200 mmol.l-1 NaCl stress.
After 24 h, the activities of these antioxidases were
maximum and subsequently decreased. Quantitative real-time
PCR analysis revealed that they were salt-inducible genes
and their transcript levels were gradually increased during
0 - 24 h and most abundant after 24 h treatment with 200
mmol.l-1 sodium chloride. Therefore, these
results from above indicated that the expressions of POD,
SOD and CAT genes were induced by NaCl; the activities of
POD, SOD and CAT were increased, which enhanced the
tolerance of oilseed oilseed rape plants against NaCl
stress.
Key
words:
Salt stress,
Brassica
napus L., anti-oxidant enzymes, quantitative
real-time PCR. |
