Short Communication

Enhanced production of glucose oxidase from UV-mutant of Aspergillus niger

Muhammad Ramzan¹ and Tahir Mehmood²

¹Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan.

²Department of Chemistry, University of Sargodha-40100, Pakistan.

*Corresponding author. E-mail: ramzanone@yahoo.com.

Accepted 16 December, 2008

UV rays were used as mutagen in wild type strain of Aspergillus niger for enhanced production of glucose oxidase. After mutangenization and selection, mutant A. niger strains, resistant to 2-deoxy-D-glucose were obtained. The mutants showed 1.57 and 1.98 fold increase in activities of extra and intra cellular glucose oxidase respectively in comparison with the parental strain. Out of 6 mutants, mutant U-6 was selected as the best producer of glucose oxidase after 36 h of fermentation using black strap molasses as substrate.

Key words: Aspergillus niger, glucose oxidase, mutagenesis, UV.