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Antagonistic bioactivity
of an endophytic bacterium isolated from Epimedium
brevicornu Maxim
Rong-lin He1, Guo-ping Wang2,
Xiao-Hong Liu1, Chu-long Zhang1*
and Fu-cheng Lin1*
1State
Key Laboratory for Rice Biology, Institute of Biotechnology,
Zhejiang University, Kaixuan Road 268, Hangzhou 310029,
China.
2Dayang
Chemical CO. Ltd., Chaoyang Road 22, Dayang town, Jiande,
311616, China.
*Corresponding author. E-mail:
clzhang@zju.edu.cn,
fuchenglin@zju.edu.cn,
Tel: +86 571 8697 1185.
Accepted
2 January, 2009 |
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Endophytic bacteria are one of the most potential biological
control agents in plant disease protection. The aim of this
work was to evaluate the antimicrobial activities of a
strain of endophytic bacterium which was isolated from
Epimedium brevicornu Maxim. The dual tests revealed that
this endophytic bacterium strain displayed a wide-spectrum
antimicrobial activity against 14 fugal phytopathogens and
one bacterial phytopathogen; it especially strongly
inhibited Alternaria alternata, Sclerotinia
sclerotiorum, Verticillium dahliae, Botrytis
cinerea and Botrytis fabae. Identification of
this strain based on morphology, physiological and chemical
characteristics and 16S rRNA gene sequence analysis
demonstrated that it belonged to the genus
Phyllobacterium.
Key
words:
Endophytic bacteria, antimicrobial bioactivity, 16S
rRNA gene. |
