|
A rapid mini-prep DNA
extraction method in rice (Oryza sativa)
Ahmadikhah, A.
Department
of Plant Breeding and Biotechonology, Gorgan University of
Agricultural Sciences and Natural Resources,
Bassij Sq., P. O. Box 4913815739,
Gorgan, Iran. E-mail:
ahmadikhah_a@gau.ac.ir.
Accepted 7
February, 2008 |
|
DNA extraction is an important step in molecular assays and
plays a vital role in obtaining high-resolution results in
gel-based systems, particularly in the case of cereals with
high content of interfering components in the early steps of
DNA extraction. Here a rapid mini-prep DNA extraction
method, optimized for rice, which was achieved via some
modifications in present DNA extraction methods, especially
in first step of cell wall lyses and the use of cheap and
frequent chemicals found in every laboratory is presented.
Normal quality and quantity was obtained by the method. The
PCR based assays also revealed the efficiency of the method.
This method is applicable with both dry and fresh samples,
does not require liquid nitrogen, and is easy, rapid and
applicable in every laboratory.
Key
words:
PCR, DNA extraction, miniprep, rice. |
