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Microbial biocatalytic
preparation of 2-furoic acid by oxidation of 2-furfuryl
alcohol and 2-furanaldehyde with Nocardia corallina
Herminia I. Pérez,*
Norberto Manjarrez, Aída Solís, Héctor Luna, Mario A.
Ramírez and Julia Cassani
Departamento de Sistemas Biológicos, Universidad Autónoma
Metropolitana Unidad Xochimilco, Calz. del Hueso No. 1100,
Col. Villa Quietud, Deleg. Coyoacán, C.P. 04960 México, D.
F., México.
*Corresponding author. E-mail:
hperez@correo.xoc.uam.mx. Tel.: (52)-55-5483-72-55 or
(52)-55-5483-73-54. Fax: (52)-55-5483-72-37.
Accepted
9 March, 2009 |
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The preparation of 2-furoic acid via oxidation of 2-furfuryl
alcohol and 2-furanaldehyde was studied by two procedures
using whole cells of Nocardia corallina B-276.
We describe a comparison using a batch cultivation (3-L)
versus resting cells in potassium phosphate buffer (0.1 M,
pH 7.0). In the 3-L bioreactor, 2-furfuryl alcohol (1.35
g/L) was biotransformed after 24 h, to the acid with 81%
yield. The bioconversion of 2-furfuryl alcohol using
resting cells leads to and almost quantitative yield (98%)
of 2-furoic acid in 21 h. 2-furanaldehyde was also oxidized
to the 2-furoic acid in 88% yield in 8 h, the ratio
substrate:cells was 1:3.5 (w/w). This method was scaled up
to 9 g/L of 2-furanaldehyde.
Key
words:
Biocatalyst, 2-furoic acid, 2-furanaldehyde, 2-furfuryl
alcohol, Nocardia corallina, oxidation. |
