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Analysis of genetic
variation among accessions of critically endangered
Rhaponticoides
iconiensis and
Rhaponticoides mykalea
based on RAPD and SDS-PAGE markers
Leyla Açık1*,
Fatma Öztürk1, Mecit Vural1, Osman
Tugay2 and I.Sefa Gürcan3
1Gazi
University, Faculty of Science, Department of Biology,
Ankara, Türkiye.
2Selcuk
University, Faculty of Science, Department of Biology,
Konya, Türkiye.
3Ankara
University, Faculty of Veterinary, Department of
Biostatistics, Ankara, Türkiye.
*Corresponding author. E-mail:
leylaacik@gmail.com. Tel.: + 90 312 2021185. Fax: + 90
312 2122279.
Accepted
2 March, 2009 |
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Rhaponticoides iconiensis (Hub.-Mor.) M.V.Agab and Greuter is a rare
and endangered endemic species of the Konya region of
Turkey. One related taxon, Rhaponticoides mykalea
(Hub.-Mor.) M.V.Agab and Greuter, is morphologically similar
but occurs in different geographical locations. This study
has been conducted on the biology of this threatened plant
in order to understand better the factors that should be
included in the development of conservation practices. The
genetic variations were studied using RAPD markers and SDS-PAGE
profiles of total seed proteins for three R. iconiensis
populations and two R. mykalea populations. The
analyzed R. iconiensis populations belonged to
different soil types (calcareous and volcanic). The analyzed
R. iconiensis and R. mykalea populations
belonged to different bioclimatic zones. A genetic diversity
within populations was detected both by SDS-PAGE and RAPD
for R. iconiensis populations. The level of variation
did not differ with respect to soil type for the species
studied. Populations collected from the same soil types
carried more polymorphisms than those grown in different
zones. The genetic diversity was revealed more clearly for
all populations by RAPD than through analyzing proteins.
Differentiation between ecological groups was higher than
that revealed within groups. Conservation programs should
take into account the level of genetic diversity within
population revealed by these markers according to soil
types.
Key
words:
Rhaponticoides iconiensis,
endangered, SDS-PAGE, RAPD. |
