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In vitro
plant regeneration from Turkish Narbon Vetch (Vicia
narbonensis L. var. narbonensis L.)
Hayrettin Kendir, Nurdan Sahin-Demirbag, Muhammad Aasim
and Khalid Mahmood Khawar*
Department
of Field Crops, Faculty of Agriculture, Ankara University,
Ankara, Turkey.
*Corresponding author. E-mail:
kmkhawar@gmail.com.
Tel: +90-312-596 15 40.
Fax:
+90-312-318 26 66.
Abbreviations: BAP,
6-Benzylaminopurine; IBA, Indole 3 butyric acid.
Accepted
2 December, 2008 |
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Narbon vetch (Vicia narbonensis L.) is an important
forage species among vetches of central Europe,
Mediterranean, Near East, Ethiopia, central Asia and India.
The study reports in vitro micropropagation of narbon
vetch using cotyledon node, shoot tip and zygotic embryo
explants on MS medium containing 0.25, 0.50, 0.75 and 1.00
mg/l BAP. The highest number of 4.42 shoots was recorded on
cotyledon node explants, which was followed by significantly
reduced number of 3.40 and 3.54 shoots per explant on shoot
tip and zygotic embryo with two cotyledons, respectively.
Zygotic embryo with two cotyledons explant was recalcitrant
and slow in regeneration compared to other explants but any
concentration of BAP was sufficient for multiple shoot
regeneration. Shoot length decreased statistically with
increase in each concentration of BAP in the regeneration
medium. 65.0% shoots rooted on MS medium containing 0.5 mg/l
IBA and newly regenerated plants were acclimatized in the
greenhouse.
Key
words:
In vitro, Narbon vetch, regeneration, Vicia
narbonensis L, shoot tip, cotyledon node, zygotic embryo
with two cotyledons. |
