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Induction of apoptosis and
cell proliferation inhibition by paclitaxel in FM3A cell
cultures
Nazlı N. Ilgar and Gül Özcan Arıcan*
İstanbul
University, Faculty of Science, Department of Biology,
İstanbul/Turkey.
*Corresponding author. E-mail:
gozcan@istanbul.edu.tr. Tel: 00 212 455 57 00 ext.
15093. Fax: 00 212 528 05 27.
Accepted
2 December, 2008 |
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In this study, anti-proliferative and apoptotic effects of
paclitaxel, which is itself an anti-chemotherapeutic agent,
to FM3A cell line originated from Mouse mammary carcinoma at
7 different doses were examined. Seven different doses of
paclitaxel (P1 = 3 nM, P2 = 7.5 nM, P3 = 15 nM, P4 = 30 nM,
P5 = 60 nM, P6 = 120 nM, P7 = 240 nM) were administered to
cells for 24 and 48 h. Growth rate measurements showed that
living cell number decreased and number of dead cells
increased (p<0.05). Acquired growing rates were supported by
mitochondrial dehydrogenase enzyme activity. Loss of volume,
protrusions at plasma mebrane (bleb formations), nuclear
condensations and fragmentations, and apoptotic body
formations were observed in cells whose morphologic criteria
were examined by phase contrast and fluorescent microscope.
According to apoptotic index rates determined by DAPI, most
intense apoptotic cell formations were observed for P2 dose,
which is accepted as critical for this cell line. DNA
fragmentations were shown by agarose gel electrophoresis
method. Acquired deductions showed that of the seven
different doses of paclitaxel, P2 (7.5 nM) was the best dose
to induce apoptosis in FM3A cells for 24 and 48 h.
Key
words:
in vitro, FM3A, cell kinetics, DNA, apoptosis,
paclitaxel. |
