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Effect of explant age,
hormones on somatic embryogenesis and production of multiple
shoot from cotyledonary leaf explants of Solanum
trilobatum L.
V.N. Chakravarthi Dhavala, Tejeswara Rao D, Yechuri V.
Rao and Prabavathi K.
Botany
Department, Andhra University, Visakhapatnam - 530003,
India.
*Corresponding author. E-mail:
raovyechuri@yahoo.co.in, Tel.: 91-0891-2844562.
Abbreviation: MS,
Murashige and Skoog; BAP, 6-benzyl adenine purine,
KN, kinetin; IBA, indole-3-butyric acid; SE,
somatic embryogenesis.
Accepted
6 January, 2009 |
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The present study examines the effect of explant age and
various concentrations of kinetin and BAP on somatic
embryogenesis and organogenesis in Solanum trilobatum
L. MS medium fortified with 11.1 µM BAP + 13.95 µM KN
produced highest frequency of embryogenesis (97.3%) and
average number of multiple shoots (48.4 ± 1.33) from
cotyledonary leaf explants at two opened leaves stage.
The presence of BAP and/or KN was critical in increasing
embryos and multiple shoots per explant with its increasing
concentrations which was also supported from significant
positive corrilation (p < 0.05). It was also observed that
embryogenic and organogenic response was significantly
affected by explant type in all concentrations of BAP and KN
alone as well as in combinations (P < 0.01).
Rooting of shoots occurred on half strength MS medium
supplemented with 7.35 and 9.8 µM IBA respectively. The
rooted plantlets were well accomplished with a survival
frequency of 86.5 ± 5%. Moreover, there were no phenotypic
differences observed between the in vitro regenerated
and in vivo plantlets.
Key
words:
Somatic embryogenesis, regeneration, endogenous hormones,
explant type, explant age, Solanum trilobatum L.
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