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Regeneration potential of
seedling explants of chilli (Capsicum annuum)
M. Ashrafuzzaman1,3*, M. M. Hossain2,
M. Razi Ismail3, M. Shahidul Haque2,
S. M. Shahidullah3 and Shahin-uz-zaman2
1Department
of Crop Botany, Bangladesh Agricultural University,
Mymensingh 2202, Bangladesh.
2Department
of Biotechnology, Bangladesh Agricultural University,
Mymensingh 2202, Bangladesh.
3Institute
of Tropical Agriculture, Universiti Putra Malaysia, 43400
Serdang, Malaysia.
*Corresponding author. E-mail:
ashraf.u.zaman@gmail.com.
Phone: +880 (0) 91 55 695 ext. 2460; GSM: + 880 (0) 171 738
8991; Fax: +880 (0) 91 55 810.
Abbreviations: BAP, 6-benzyle amino purine; NAA,
a-naphthalene
acetic acid; IBA, indole-butyric acid; IAA,
indole-3-acetic acid; MS, Murashige and Skoog’s
(1962) medium.
Accepted
27 January, 2009 |
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A study was conducted with hypocotyl, cotyledon and shoot tip
of chilli as explants for regeneration on MS medium
supplemented with different concentrations and combinations
of auxins and cytokinins. Regeneration potential was
determined by two ways. One is regeneration of shoot via
callus formation from hypocotyls and cotyledon explants;
another was direct shoot regeneration from shoot tip explant.
The highest callus was induced from hypocotyl in a
combination of BAP (5.0 mgL-1) with NAA (0.1 mgL-1)
and cotyledon in a combination of BAP (5.0 mgL-1)
with IAA ((1.0 mgL-1). The callus induction as
well as shoot initiation was higher in hypocotyls than
cotyledon. Shoot tips regenerated into plantlets directly
with sporadic small callus at the base. Shoot elongation was
accelerated by using additional supplementation of GA3
and AgNO3. Regenerated shoots rooted best on the
MS medium supplemented with 0.1 mg L-1 NAA + 0.05
mg L-1 IBA.
Key words:
Chilli,
hypocotyl, cotyledon, shoot tip,
explants, hormone. |
