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Laboratory based
degradation of light crude oil by aquatic phycomycetes
Yerima, M. B.1*, Balogun, A. A.1,
Farouq, A. A.1 and Muhammad, S.2
1Microbiology
Unit, Faculty of Sciences, Usmanu Danfodiyo, University,
Sokoto, P. M. B. 2346, Sokoto State Nigeria.
2Botany
Unit, Faculty of Sciences, Usmanu Danfodiyo, University,
Sokoto P. M. B. 2346, Sokoto State, Nigeria.
*Corresponding
author. E-mail:
yerimbel@yahoo.co.uk
Accepted 8
July, 2005 |
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Water samples were collected from Dundaye river (an offshoot
of sokoto river Rima) using sterile conical flasks. The
samples were immediately transported to the Microbiology
Laboratory and introduced into petri dishes containing hemp
seeds (Crotalaria juncea) and allowed to stand
on the bench. The hemp seeds served as baits, after five
days a whitish cottony growth surrounding the seeds was
observed. The growth was subcultured in potato dextrose
broth (PDB) and incubated at 37°C for 48 h. In the
degradation experiment, a minimal medium containing 0.2% of
the crude oil as sole source of carbon was prepared in
replicates. The results obtained indicate that phycomycetes
have a potential use in bioremediation.
Key
words:
Aquatic phycomycetes, crude oil. |
