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Pheno- and genotyping of
Pasteurella multocida isolated from goat in Iran
Jalal Shayegh1*, Jalil Dolgari Sharaf1,
Peyman Mikaili2 and Hadi Namvar1
1Department
of Veterinary Medicine, Islamic Azad University (IAU) of
Shabestar, Shabestar, Iran.
2Department
of
Pharmacology, Urmia University of Medical Sciences, Urmia,
Iran.
*Corresponding author. E-mail:
jalal_shayeghi@yahoo.co.in.
Accepted 15
May, 2009 |
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In this study, phenotype, capsular type and some virulence
factors (pfha1, tbpA, and toxA) of caprine Pasteurella
multocida were described in 10 isolates from goat. Two
biochemical biovars were recognized among the isolates based
on dulcitol and sorbitol fermentation. The isolates belonged
to biovars P. multocida sub. multocida (80%) and
P. multocida sub. septica (20%). Capsular typing of
isolates by PCR demonstrated two capsular types A (90%) and
D. Among 3 virulence genes detected by PCR, we found a
remarkable high prevalence of tbpA (80%) and toxA (70%)
genes among caprine isolates. The high prevalence of these
genes owning association of disease status among healthy
animals showed high potential of the strains in induction of
disease. The high prevalence of toxA and tbpA among goat is
very similar to the ones in sheep isolates. Comparison of
virulence genes profile showed possibility of P.
multocida transmission between sheep and goat.
Key
words:
Pasteurella multocida, goat, typing, Iran. |
