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Full Length Research Paper
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Differentiation of
Lactobacillus-probiotic strains by visual comparison of
random amplified polymorphic DNA (RAPD) profiles
Norlida Abdul Manan1, Sieo Chin Chin1,2*,
Norhani Abdullah1,2 and Ho Yin Wan1
1Institute
of Bioscience, 43400 UPM Serdang, Selangor Darul Ehsan,
Malaysia.
2Department
of Microbiology, Faculty of Biotechnology and Biomolecular
Sciences, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor Darul Ehsan, Malaysia.
*Corresponding author. E-mail:
ccsieo@biotech.upm.edu.my. Tel.: +603-89466702. Fax:
+603-89430913.
Accepted 3
July, 2009 |
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Abstract |
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In the present study, distinctive RAPD fingerprints were
generated for 12 Lactobacillus-probiotic
strains from 5 Lactobacillus species (L. brevis,
L. reuteri, L. gallinarium, L. salivarius
and L. panis) after optimization of the RAPD
parameters such as MgCl2, Taq polymerase, primer
concentration and type of primer. The strains were
differentiated under the same PCR protocol but different
concentration of primer OPM-05 (50 pmole to differentiate
the 5 L. brevis strains and 75 pmole to differentiate
2 strains of L. gallinarium, 3 strains of L.
reuteri, a strain of L. panis and L.
salivarius). The RAPD fingerprints generated could be
differentiated by visual comparison of the profiles, without
being analysed by relevant softwares. This allows specific,
rapid, immediate and convenient identification of the
Lactobacillus strains.
Key
words:
Lactobacillus, RAPD analysis, differentiation. |
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