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Genetic diversity analysis
of the species of Gossypium by using RAPD markers
Zahid Mahmood1, Farah Raheel1,
Altaf A Dasti1, Saima Shahzadi1,
Mohammad Athar2* and Manzar Qayyum3
1Institute
of Pure and Applied Biology, Bahauddin Zakariya University,
Multan-60800. Pakistan.
2California
Department of Food and Agriculture, 3288 Meadowview Road,
Sacramento, CA 95832, USA.
3Infinity
Engineering and Technology Corporation, Research,
Development and Extension Division, 4810 Black Rock Drive,
Sacramento, CA 95835, USA.
*Corresponding author. E-mail:
atariq@cdfa.ca.gov.
Accepted
31 March, 2009 |
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Random Amplified Polymorphic DNAs (RAPDs), a DNA polymorphism
assay based on the amplification of random DNA segments with
single primers of arbitrary nucleotide sequence, was
utilized to measure the genetic distance among the species
of Gossypium. Variations among 20 different species
of Gossypium were analyzed with 63 random 10-mer
primers using the polymerase chain reaction (PCR). Out of a
total of 370 RAPD bands generated from the 20 wild species,
310 were perceived polymorphic. The study detected a high
level of genomic variability of the species of Gossypium.
The maximum similarity of 64% was observed between
Gossypium arboreum and Gossypium herbaceum and
minimum similarity of 3% was observed between Gossypium
stocksii and Gossypium longicalyx. Cluster
analysis by the unweighted pair group method of arithmetic
means (UPGMA) showed that 20 wild species can be divided
into several groups, although it is believed that
Gossypium is a very widely distributed, wild species
having being discovered in all the continents of the world
which extends into the subtropical region. The relationship
among the species is presented in the dendrogram.
Key
words:
Random amplified polymorphic DNA, polymerase chain reaction,
wild species, genetic diversity, Gossypium wild
species. |
