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Cryopreservation of seeds
of lily [Lilium Iedebourii (Baker) Bioss]: Use of
sucrose and dehydration
Behzad Kaviani*, Davood Hashem Abadi, Ali
Mohammadi Torkashvand and Shahram Sedaghat Hoor
Department
of Horticultural Science, Faculty of Agriculture, Islamic
Azad University, Rasht Branch, Rasht, Iran.
*Corresponding author. E-mail:
b.kaviani@yahoo.com.
Tel: 0989111777482, 00981314240149. Fax: 00981313462255.
Accepted 20
March, 2009 |
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Cryopreservation of germplasm at liquid nitrogen (-196°C) is
a perfect method for the long-term conservation of plant
genetic resources. A cryopreservation process using
dehydration was performed for seeds of lily [Lilium
Iedebourii (Baker) Bioss]. Seeds were subjected to a
rapid freezing protocol in liquid nitrogen following
dehydration and treatment with 0.75 M sucrose for 1 h.
Survival after freezing was nil for control seeds and 75%
for seeds treated with sucrose and dehydration.
Key
words:
Dehydration, germplasm conservation, lily and sucrose. |
