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Studies on antioxidant and
antiradical activities of Dolichos biflorus seed
extract
Bibhabasu Hazra, Rhitajit Sarkar, Sourav Mandal, Santanu
Biswas and Nripendranath Mandal*
Division of Molecular Medicine, Bose Institute, P-1/12
CIT Scheme VIIM, Kolkata-700054, India.
*Corresponding author. E-mail:
mandaln@rediffmail.com or
nripen@boseinst.ernet.in. Tel.: +91-33-25693285. Fax:
+91-33-23553886.
Accepted 5
June, 2009 |
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The antioxidant potential of 70% methanolic extract of
Dolichos biflorus seeds was assessed using different
tests including total antioxidant activity, hydroxyl radical
scavenging, superoxide radical scavenging, nitric oxide
radical scavenging, hypochlorous acid scavenging, reducing
power and lipid peroxidation. The total phenolic and
flavonoid contents were also evaluated. The extract was
found to be an antioxidant with a trolox equivalent
antioxidant capacity (TEAC) value 0.28 ± 0.006, the extract
has shown its scavenging activity for different radicals and
184.55 ± 7.08, 114.14 ± 6.85, 74.77 ± 1.73, 287.9 ± 8.56
µg/ml were determined as IC50 value for hydroxyl,
superoxide, nitric oxide and hypochlorous acid respectively.
The extract has shown moderate inhibition of lipid
peroxidation with IC50 128.63 ± 3.44
μg/ml. It
was also observed that the plant extract (100 mg) has 44.67
± 0.004 mg/ml gallic acid equivalent phenolic and 72.0 ±
0.008 mg/ml quercetin equivalent flavonoid content. The
present results demonstrate D. biflorus seeds as a
potential source of natural antioxidant.
Key
words:
Antioxidant, Dolichos biflorus, flavonoid content,
free radical, phenolic content, TEAC. |
