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Direct in vitro
regeneration of a medicinal tree Oroxylum indicum
(L.) Vent. through tissue culture
M. Gokhale and Y. K. Bansal*
Plant
Tissue Culture Lab., Department of Biosciences, R. D.
University, Jabalpur 482001 (M. P.), India.
*Corresponding author.
E-mail:
yogendrakbansal@rediffmail.com.
Tel.:0761-2604060 or 09425155199. Fax: 0761-2603752.
Accepted 2
June, 2009 |
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A simple and reliable protocol was developed through apical
and axillary bud explants of Oroxylum indicum for
multiple shoot regeneration. Effect of 2 cytokinins; BAP and
KN, was studied. BAP at 4.43 µM proved better than KN with
highest frequency of shoot initiation and maximum number of
shoots initiated. Axillary bud showed significantly (p <
0.05) high shoot multiplication on MS medium with 4.43 µM
BAP in subsequent sub culture passages. Elongated shoots
were rooted on half strength MS medium with 4.92 µM IBA. Of
the various methods used for hardening of in vitro
regenerated plantlets, maximum plantlets survived after
their subsequent transfer through sterilized-distelled-tap
water and ultimately into soil:sand mixure.
Key
words:
Apical bud, axillary bud, 6-benzylaminopurine, in vitro,
tissue cultue, Oroxylum indicum L. |
