Full Length Research Paper

Effects of plant growth regulators on callus induction from Cananga odorata flower petal explant

Z. Nurazah*, M. Radzali, A. Syahida and M. Maziah

Natural Product Laboratory, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

*Corresponding author. E-mail: nurazahzain@yahoo.com.

Accepted 24 February, 2009

The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine (BAP) as the plant growth regulators. It was observed that the C. odorata callus could be induced on media containing the combination of 3 mg/L NAA and 0.5 mg/L BAP. High concentration of NAA gave rise to pale, whitish and friable callus after 1 - 2 weeks of culture. The optimum pH for the cell culture was about 5.7 and incubation at 25 ± 2°C, in totally dark condition.

Key words: Cananga odorata, 1-naphtalene acetic acid, 6-benzylaminopurine, callus culture.