of an in vitro culture system adapted to banana
Marie Chantal Koffi1, Ivan Enrique de la
Providencia1, Annemie Elsen2 and
catholique de Louvain (UCL), Unité de microbiologie, Place
croix du Sud 3, 1348 Louvain-la-Neuve, Belgium.
of Tropical Crop Improvement; Division of Crop Biotechnics,
Faculty of Bioscience Engineering, K. U. Leuven, Kasteelpark
Arenberg 13, 3001 Leuven, Belgium.
*Corresponding author. E-mail:
firstname.lastname@example.org. Tel.: + 32 (10) 47 46 44.
Fax: +32 (10) 45 15 01.
Arbuscular mycorrhizal; BCA, biological control
agent; MSR, modified Strullu Romand.
Accepted 6 March, 2009
The beneficial impact of arbuscular mycorrhizal (AM) fungi
on banana nutrition and resistance against abiotic and
biotic stresses is well documented. However, most studies
were conducted under greenhouse or field conditions and none
reported the life cycle of the AM fungi on banana roots. It
is obvious that any system associating both organisms under
strict controlled in vitro culture conditions may
help to comprehend the role of AM fungi in banana
physiology. Here we developed an in vitro culture
system associating autotrophic micropropagated banana plants
with an AM fungus (Glomus intraradices). Intraradical
root colonization, with production of arbuscules and
vesicules, as well as extraradical development with
production of new spores was observed. This study opens the
door to investigate the role of AM fungi in banana
physiology in particular for the control (through e.g. the
elicitation of defence genes in bananas) of major banana
root pathogens under strict in vitro culture
Arbuscular mycorrhizal fungi, Musa acuminata, in
vitro conditions, sporulation.