|
Antifungal activity of
extracts and phenolic compounds from Barringtonia
racemosa L. (Lecythidaceae)
N. M. Hussin*, R. Muse, S. Ahmad, J. Ramli, M. Mahmood,
M. R. Sulaiman, M. Y. A. Shukor, M. F. A. Rahman and K. N.
K. Aziz
Department
of Biochemistry, Faculty of Biotechnology and Biomolecular
Sciences, University Putra Malaysia, 43400 UPM Serdang,
Selangor, Malaysia.
*Corresponding author. E-mail:
yamasyikav@yahoo.com.
Tel.: 603-82100644.
Fax: 603-87244757.
Accepted 20
April, 2009 |
|
The antifungal activity of methanolic, ethanolic and boiling
water extracts of Barringtonia racemosa leaves,
sticks and barks were investigate against Fusarium
sp., Tricoderma koningii, Penicillium sp.,
Ganoderma tropicum, Ganoderma lucidum, Aspergillus sp.
and Rhizopus sp. at concentration of 50 mg/ml. Better
antifungal activity was observed with the methanolic
extracts in all aerial parts of B. racemosa that
showed excellent inhibitory activity against all the fungi
tested. The strongest inhibitory activity effect was
observed with the methanolic extract of leaf against
Fusarium sp. (53.45%), G. lucidum (34.57%),
Aspergillus sp. (32.27%) and T. koningii
(20.99%). Remarkable are also the specific effects of the
boiling water extract of leaf against Fusarium sp.
(51.72%) and with the ethanolic extract of bark against
Rhizopus sp. (37.50%). None of the boiling water
extracts of leaf, stick and bark showed inhibitory activity
effect against G. tropicum and T. koningii.
Among different fungi tested, Fusarium sp. was found
to be more sensitive to B. racemosa extracts when
compared to others. The increase in the production of
phenolics in the extracts can be correlated with the
induction of resistance in treated plant against
phytopathogenic fungi. HPLC analysis of the extract of B.
racemosa (leaves, sticks and barks) showed two different
phenolic acids (gallic acid and ferrulic acid) and four
different flavonoids (naringin, rutin, luteolin and
kaempferol). The results of present study provide scientific
basis for the use of the plant extract in the future
development as antifungal, antibacterial, antioxidant and
anti-inflammatory agent.
Key
words:
Barringtonia racemosa, antifungal, HPLC, phenolic
acids, flavonoids. |
