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An effective and low-cost
method for DNA extraction from herbal drugs of Rheum
tanguticum (polygonaceae)
Yanping Hu1,2, Xiaolong Xie1,2, Li
Wang1, Jian Yang1,2, Huaigang Zhang1*
and Yi Li1*
1Key
Laboratory of Adaptation and Evolution of Plateau Biota，Northwest
Institute of Plateau Biology, Chinese Academy of Sciences,
Xining, 810001, P. R. China.
2Graduate
University of Chinese Academy of Sciences, Beijing, 100049,
P. R. China.
*Corresponding authors. E-mail:
liyi@nwipb.ac.cn,
Phone: +86-9716101454;
hgzhang@nwipb.ac.cn; Phone: +86-9716143630.
Abbreviations: CTAB,
hexadecyltrimetylammonium bromide; ISSR, inter-simple
sequence repeats.
Accepted 26
March, 2009 |
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Rhubarb is an important traditional Chinese herbal drug with
high secondary metabolites that interfere with DNA
extraction procedures and downstream applications, such as
DNA restriction and amplification. An effective and low-cost
protocol for isolating genomic DNA from root of Rheum
tanguticum is described in this paper. It involved a
modified CTAB method with distilled water pretreating
samples. The A260/A280 absorbance
ratio of extracted DNA, free from polysaccharide,
polyphenols and tannins contaminants, ranged from 1.85 to
2.0 within the high level of purity. The DNA preparations
were found suitable for analyses with restriction enzyme
digestion and inter-simple sequence repeats (ISSR). This
method is helpful for further studies on rhubarb at
molecular level and is more cost-effective than commercial
kit.
Key
words:
Rhubarb, Rheum tanguticum, DNA extraction, ISSR,
secondary metabolites. |
