|
Molecular characterization
of 26S proteasome regulatory subunit in dermatophyte
pathogen Trichophyton verrucosum
Moradali Fouladvand1, Behrouz Naeimi1,2*,
Sassan Rezaie3, Fatemeh Noorbakhsh4
and Mohammad Reza Safari3
Department
of Microbiology and Parasitology, Faculty of medicin,
Bushehr 2University of Medical Sciences, Bushehr,
P. O. Box: 3631, Iran.
The
Persian Gulf Tropical and Infectious disease Research
Centre, Bushehr University of Medical Sciences, Bushehr,
Iran.
3Division
of Molecular Biology, Department of Medical Mycology and
Parasitology, School of Public Health, Tehran University of
Medical Sciences, Tehran, Iran.
4Islamic
Azad University, Varamin-pishva Branch, Varamin, Iran.
*Corresponding author.
E-mail:
b.naeimi1350@gmail.com. Tel: +98-912-3617933.
Fax: +98-771-2528724.
Accepted 7
April, 2009 |
|
Trichophyton verrucosum is a zoophilic dermatophyte, which causes
dermatophytosis infection in human as well as animals. 26S
proteasome is an important protein in eukaryotic cells that
is involved with
degradation of unneeded or damaged
proteins, when tagged with ubiquitin. In this study, we characterized the 26S Proteasome regulatory subunit
gene in dermatophyte pathogen T. verrucosum. High
molecular weight DNA has been isolated from T. verrucosum
and utilized with pairs of specific nucleotides primers,
designed from highly preserved regions of the 26S proteasome
regulatory subunit genes. Obtained DNA fragments were
sequenced and the results were analyzed in GenBank. This DNA
fragment, which contains no intron within its open reading
frame, encodes a polypeptide with 332 amino acids. The
characterized PCR fragments revealed significant homology
with other 26S proteasome regulatory subunit genes in
GenBank.
Key
words:
Dermatophyte,
Trichophyton verrucosum,
fungal DNA, nucleic acid sequencing,
26S proteasome. |
