Mapping of quantitative
trait loci controlling Orobanche foetida Poir.
resistance in faba bean (Vicia faba L.)
Díaz-Ruiz R.1, Torres A.2,
Gutierrez M. V.2, Rubiales D.3, Cubero
J. I.4, Kharrat M.5, Satovic Z.6
and Román B.2*
de Postgraduados-Campus Puebla, Km 125.5 Carretera Federal
Méx-Pue. C. P. 72760, Puebla, Apdo 2-12, México.
(Junta de Andalucía), CIFA “Alameda del Obispo”, Área de
Mejora y Biotecnología, Apdo. 3092, 14080 Córdoba, Spain.
de Agricultura Sostenible, CSIC, Apdo. 4084, 14080 Córdoba,
de Genética, ETSIAM, Campus de Rabanales, Ed. Gregor Mendel,
Universidad de Córdoba, 14071 Córdoba, Spain.
Food Legume Laboratory, INRAT, BP 10, 2049, Ariana, Tunisia.
of Seed Science and Technology, Faculty of Agriculture,
University of Zagreb, Svetosimunska 25, 10000 Zagreb,
email@example.com. Tel: 34 957 016125.
Fax: 34 957 016043.
26 December, 2008
Although in the Mediterranean region the most important
Orobanche weed attacking leguminous crop is Orobanche
crenata, recently Orobanche foetida has been
found attacking faba bean, vetch and chickpea in Tunisia and
vetch in Morocco. In this study we have identified and map
the quantitative trait loci (QTL) controlling resistance to
O. foetida in faba bean (Vicia faba) and
studied their stability in two different environments. One
hundred and forty four Recombinant Inbred Lines (RILs)
derived from the cross between a susceptible and a resistant
parent were analysed using isozymes, RAPD, seed protein
genes, STSs, ESTs, microsatellites and SCAR markers. Two
hundred and seventy seven markers segregating in the RIL
population could be mapped into 21 linkage groups, 9 of them
assigned to specific chromosomes. By evaluating broomrape
resistance in these RILs under field conditions, two QTLs
for O. foetida resistance were identified showing
instability across environments. Whereas Of1 was located in
the chromosome 1 and explained 7% of the phenotypic
variation in one environment, Of2 was assigned to chromosome
3, was only identified in the second environment and
explained 9% of the trait variation. Since this map has been
used before to locate O. crenata resistance QTLs its
exploitation offers the possibility of accumulating
resistance genes in V. faba germplasm against both
Orobanche foetida, Vicia faba, parasitic
plants, resistance QTLs, molecular mapping.