Full Length Research Paper

Construction and analysis of a cDNA library from yellow-fruit ginseng (Panax ginseng C.A.Meyer.) leaf tissue

Chengjun Yang^1,2, Jun Wang^2,3*, Chuanping Yang² and Guifeng Liu²

¹Postdoctoral Research Centres of Biology, Northeast Forestry University, Harbin 150040, P. R. China.

²Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, College of forestry, Northeast Forestry University, Harbin 150040, P. R. China.

³College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P. R. China.

*Corresponding author. E-mail: junwang1966@yahoo.com.cn. 

Tel: 86-045-82191829(O). Fax: 86-451-82190607-11.

Accepted 9 July, 2009

   Abstract

The total RNA was isolated from yellow-fruit ginseng (Panax ginseng C.A. Meyer) leaf tissue. A cDNA library of panax ginseng leaves was constructed by using pDNR-LIB vector according to the SMART cDNA library construction kit protocol. We obtained 378 high quality sequences (GenBank accession number: ES672876-ES673253). ESTs were annotated, analyzed by BlastX and functional classified based on gene ontology, the results showed that 221 ESTs showed significant similarities to gene sequences in Nr database and were known genes, 21 ESTs were non-significance and unknown function genes, and 136 ESTs were considered novel genes. Most of the ESTs appeared to be related to physiological and cellular processes.

Key words: cDNA library, expressed sequence tags (EST), Panax ginseng.