|
A plant defensin gene from
Orychophragmus violaceus can improve Brassica napus’
resistance to Sclerotinia sclerotiorum
Jun Wu, Lin-tao Wu, Zhi-bin Liu, Lei
Qian, Mao-hua Wang, Li-rong Zhou, Yi Yang, Xu-feng Li*
Key
Laboratory of Bio-resources and Eco-environment, Ministry of
Education, College of Life Science, Sichuan University,
Chengdu, Sichuan 610064, P.R. China.
*Corresponding author.
E-mail:
lixufeng08@gmail.com. Tel: 86-28-85412281.
Fax: 86-28-85410957.
Accepted 7 October, 2009 |
|
A plant
defensin gene, named as Ovd, was cloned from
Orychophragmus violaceus (L.) O. E. Schulz and
subsequently introduced into Brassica napus genome by
Agrobacterium tumefaciens-mediated transformation
method in sense and antisense forms under the control of the
CaMV 35S promoter. Genomic PCR analysis confirmed the
integration of the sense and antisense Ovd into the host
genome. Quantitative RT-PCR showed that the expression of
Ovd in the sense plant line was stronger than
non-transformed plant and antisense plant. The lesion size
on detached leaves of the transgenic plants and
nontransformed control caused by Sclerotinia sclerotiorum
mycelia was examined. Lesion size was reduced in sense
transgenic plants compared to nontransformed control (15 -
20% reduction area), while it was enlarged in antisense
transgenic plants compared to nontransformed control (14 -
20% enlargement). The results showed that the
over-expressing transgenic B. napus had higher
resistance to S. sclerotiorum.
Key words:
Brassica napus, Orychophragmus violaceus,
plant defensin, Sclerotinia sclerotiorum,
transformation. |
