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Study on genetic
variability of Cassidula aurisfelis (snail) by random
amplified polymorphic DNAs
Wan Bayani, W. O.,
Mohammad, Shafie S.* and Zaleha, K.
Department of Biological Sciences, Faculty of Science
and Technology, University Malaysia Terengganu, 21030
Mengabang Telipot, Kuala Terengganu, Malaysia.
*Corresponding author. E-mail:
msshafie@yahoo.com.my.
Accepted
15 May, 2008 |
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The genetic
variability among individuals of Cassidula aurisfelis
from Setiu Wetland, Terengganu Darul Iman was examined by
using the random amplified polymorphic DNA (RAPD) technique.
Ten oligonucleotide primers were screened and three primers
were selected (OPA 02, OPA 04 and OPA 10) to amplify DNA
from five samples of C. aurisfelis from Setiu
Wetland, Terengganu. A total of 28 RAPD bands (RAPDs) with
17 polymorphic bands (60.71%) with size ranging from 300 –
1750 bp were scored from the population. Genetic distance
for samples ranges from 0.135 to 0.269. Similarity index of
samples ranges from 0.7179 to 0.8649 (mean 0.7810 ± 0.0497).
Key
words:
Cassidula aurisfelis, snail, genomic DNA, genetic
variation, RAPD. |
