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High
frequency induction of somatic embryos and plantlet
regeneration from nodal explants of Hygrophila spinosa
T. Anders
A. Varshney,
A. Shahzad and M. Anis*
Plant Biotechnology Laboratory, Department of Botany,
Aligarh Muslim University, Aligarh 202 002, India.
*Corresponding author. E-mail:
anism1@rediffmail.com
or
shahzadanwar@rediffmail.com.
Accepted 11 May, 2009 |
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An efficient protocol is described for the rapid in vitro
plant regeneration of a medicinally important plant,
Hygrophila spinosa through direct somatic embryogenesis
from nodal explants excised from 4 week old aseptic
seedlings. Somatic embryos differentiated directly from
nodal explants on Murashige and Skoog (MS) medium
supplemented with 1.0 µM 6- benzyladenine (BA) after 1 week
of culture. The highest number (110.40 ± 3.55) of somatic
embryos were recorded on MS medium supplemented with 1.0 µM
BA and 0.5 µM NAA, growth regulator free half strength MS
medium was found suitable for maturation and conversion of
somatic embryos into complete plantlets. The well developed
in vitro regenerated plantlets were successfully
established in pots containing garden soil and grown in a
greenhouse with 85% survival rate. The regenerants present
an appearance similar to the seedlings obtained from normal
seeds. The described method can be successfully employed for
large scale multiplication and long term in vitro
conservation of H. spinosa.
Key words:
Acanthaceae, Hygrophila spinosa, somatic
embryogenesis, medicinal plant, conservation,
micropropagation. |
