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Full Length Research Paper
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Effect of alkaline
hydrolysis on the quantity of extractable protein fractions
(prolamin, albumin, globulin and glutelin) in Jatropha
curcas seed cake
Usman, L. A.*, Ameen, O.
M., Lawal A. and Awolola, G. V.
Department
of Chemistry, University of Ilorin, Ilorin, Kwara State,
Nigeria.
*Corresponding author. E-mail:
usmanlamidi@yahoo.com.
Accepted
18 June, 2008 |
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Abstract |
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Jatropha
curcas
seeds were processed and defatted to obtain the seed cake.
60 g of them were separately moistened in 0.1 – 0.5 M NaOH
and Ca(OH)2 solutions and autoclaved. Each of the
autoclaved samples was separately divided into three
portions. A portion was washed with ethanol, another with
water and the third was left unwashed. Prolamin, albumin,
globulin and glutelin fractions were extracted from the
treated and untreated seed cakes and subsequently
quantified. Untreated seed cake sample express 0.11 g/Kg
prolamin, 4.06 g/Kg albumin, 4.23 g/Kg globulin and 10.91
g/Kg glutelin of seed proteins. In Ca(OH)2
treated cake, albumin and globulin had their maximum yield
in ethanol washed cake samples. The quantities obtained were
3.95 and 5.11 g/Kg of the seed protein, respectively.
Prolamin and glutelin had their maximum yield in unwashed
and water washed cake samples. The yields were 4.84 and 9.27
g/Kg of the seed protein, respectively. In NaOH treated seed
cake, the four fractions had their maximum yield in water
washed cake. The quantities were 3.37, and 3.64 g/Kg of seed
protein for prolamin and albumin. The quantities of globulin
and glutelin were 4.90 and 9.26 g/Kg of seed proteins.
Quantity of prolamin increased in all the treated cake while
the quantities of albumin, globulin and glutelin were
reduced by the treatments.
Key
words:
Prolamin, albumin, globulin, glutelin, alkaline hydrolysis. |
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