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Isolation of bacteria from
mechanic workshops’ soil environment contaminated with used
engine oil
T. K. C. Udeani*, A . A.
Obroh, C .N. Okwuosa, P. U. Achukwu and N. Azubike
Department of Medical Laboratory Sciences, College of
Medicine, University of Nigeria, Enugu Campus, Enugu,
Nigeria.
*Corresponding
author. E-mail:
theoka2002@yahoo.com.
Accepted
28 May, 2008 |
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The study
was designed to evaluate the bacterial diversity of soil
environment contaminated with used engine oil. Ten mechanic
workshops within Enugu metropolis were selected and six soil
samples were collected from each site. These were analyzed
using Bushnell Haas enrichment medium. Samples were
enumerated using ten fold dilutions from 1:10 to 1:100000
from the soil samples. The result showed the isolation of
Bacillus Stearothermophilus (8.3%) and Cyanobacteria
(1.7%) from the sites sampled. The number of viable
bacterial growth of B. Stearothermophilus and
Cyanobacteria were enumerated and expressed in colony
forming units. Agbani had bacteria densities of 5 x 104,
1.25 x 104 and 6.25 x 105 from the
three different sites respectively, Asata with 5.10 x 104
and 2.5 x 104, independence layout with bacterial
density of 2.5 x 102 and Uwani/Coal camp with 2.5
x 104 and 6.25 x 105. Amongst the 10
different sites studied, Agbani and Uwani/Coal camp had the
highest bacteria density of 6.25 x 105.
Key
words:
Used engine oil, contaminated soil, pollution. |
