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The
potential lipolysis function of musclin and its mRNA
expression both in Pig adipose tissue and primary adipocyte
Chao Sun*,
Yongjia Peng,
Dongfeng Jiang
and Zhongpin Zhang
College
of Animal Science and Technology, Northwest A and F
University, Yang Ling 712100, Shaanxi, China.
*Corresponding author. E-mail:
mdsys4439@hotmail.com.
Tel.:+86-29-87092164. Fax: +86-29-87092164.
Abbreviations:
RT-PCR,
Reverse transcription polymerase chain reaction;
FAS,
fatty acid synthesis; PPARγ, peroxisome proliferator
- activated receptor γ; LPL, lipoprotein lipase;
TGH,
triacyl glycerol hydrolase.
Accepted
3 April, 2009 |
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Musclin
is a newly discovered factor and its functions remain to be
defined. This study investigated the tissue expression
pattern of musclin gene and its potential effect on lipid
metabolism. Musclin mRNA levels in adipose, muscle tissues
and primary adipocytes were examined by quantitative PCR.
The musclin gene expression in adult adipose tissue was
significantly higher than that in muscle (p < 0.05), and its
expression in young adipose tissue was higher than old one.
We further found that in adipose tissue, the expression
level of musclin had a negative correlation with FAS
gene (p = 0.01), and positive correlation with PPARγ gene (p
< 0.05). In adipocyte, the expression level of musclin gene
had a positive correlation with LPL gene (p < 0.01).
Our results suggested that musclin is a potential factor
that might play an important role in the regulation of
adipogenesis.
Key
words:
Musclin, adipocyte, adipose tissue, lipid metabolism,
expression. |
