Full Length Research Paper

Morphological, molecular and biochemical evaluation of Egyptian jasmine rice variety and its M₅ derived mutants

Said M. Shehata^1,2, Megahed H. Ammar², Amr F. Abdelkalik²* and Basuny A. Zayed²

¹Ismailia Agricultural Research Station, Ismailia.

²Rice Research and Training Center, Sakha, Kafr El-Shiekh.

*Corresponding author. E-mail: aabdelkhalik@gmail.com.

Accepted 28 September, 2009

   Abstract

The current investigation was carried out at Sirw, northern part of Egypt, during 2007 and 2008 seasons. The present study mainly aimed to evaluate the morphological and molecular variation among Egyptian Jasmine and its 10 M5 derived mutants under saline soil conditions. Some mutant lines have the advantage of early maturation nearly one month earlier compared with the original variety Egyptian Jasmine. In addition, other derived mutants significantly surpassed the Egyptian Jasmine in terms of yield and its components. The biomass and grain yield recorded the highest value of expected genetic advance. The values of heritability were high for all yields and yield attributes. Some morphological traits were utilized in order to identify the M₅ morphology. DNA Markers namely Short Sequence Repeats (SSR) and Intron Splice Junction (ISJ) were used to reveal the molecular variations at molecular level among all entries. A significant level of polymorphism based on morphological and molecular levels was observed. The overall evaluation for the newly developed lines revealed that the best line was in Jasmine Sirw Line No. 3 and followed equally by Jasmine Sirw Line Nos. 8 and 9.

Key words: Jasmine rice, induced mutation, DNA markers.