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Plesiomonas
shigelloides in
stool samples of patients in the Venda Region: Possible
considerations on pathogenicity and antibiogram profiles
Ramalivhana, N. J.1
and Obi, C. L.2*
1College
of Agriculture and Environmental Sciences, University of
South Africa, P.O. Box 392, Pretoria. 0003, South Africa.
2Academic
and Research Directorate, Walter Sisulu University, Nelson
Mandela Drive, Mthatha, Eastern Cape, South Africa.
*Corresponding author. E-mail:
c355251@yahoo.com.
Accepted 18 April, 2008 |
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This study
determined the haemolytic,
haemagglutinating and antibiotic susceptibility activities
of Plesiomonas shigelloides isolated
from stool samples of patients attending different health
centers in the Venda region of South Africa.
P. shigelloides was isolated and
identified using the API 20E, API 20NE systems. Antibiotic
susceptibility profiles of the isolates were determined
using the disc diffusion method and analyzed according to
NCCLS standards. The hemolytic and hemagglutination
activities of the isolates on human, sheep, pig and chicken
red blood cells were determined using the plate and slide
methods. A total of 89 (13%)
P.
shigelloide were isolated from 660
samples. The hemolytic activities of the isolates were
variable with no heamolysis on sheep red cells. 33 (37%) of
isolates were beta lactamase producers. There was a high
level of resistance to the penicilllins with 100% resistance
to Penicillin G, Amoxicillin and Ampicillin. This study has
demonstrated multiple resistance to different antibiotics
and production of beta lactamase. Most of the isolates
showed evidence of pathogenicity as demonstrated by
hemolytic and haemagglutinating activities.
Key
words:
Plesiomonas shigelloides, stool samples, antibiograms,
red blood cells, pathogenicity, haemolysis,
haemagglutination. |
