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The effects of different
indole-3-butyric acid (IBA) concentrations, two light
regimes of in vitro rooting and acclimatization of
in vitro teak (Tectona grandis L.f) plantlets
Nor Aini, A. S.1,2*, Goh, B. L.2
and Ridzuan, R.1
1Laboratory
of Sustainable of Bioresources Management, Institute of
Tropical Forestry and Forests Products (INTROP), Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
2Department
of Forest Production, Faculty of Forestry, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
*Corresponding author. E-mail:
w.ridzuan85@yahoo.com.
Accepted 15
June, 2009 |
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Effects of different indole-3-butyric (IBA) concentrations
(0, 0.5, 1.0, 2.0, 3.0 and 5.0 mg/l), two light regimes of
in vitro rooting and acclimatization on in vitro
teak (Tectona grandis L.f) were investigated. Shoots
incubated in the light produced higher mean number of roots
(2.0) and mean root length of 15.0 and 4.5 mm when
supplemented with low IBA concentrations of 0.5 and 1.0
mg/l, respectively. Conversely, when shoots were incubated
in the dark, higher mean number of roots (8.0 and 3.0) and
mean root lengths (14.0 and 8.8 mm) were produced when
supplemented with higher IBA concentrations of 2.0 and 3.0
mg/l, respectively. IBA was found necessary for root
induction and growth in light and dark where 2.0 mg/l was
optimum as it recorded high mean number of root (6.0 and
8.0) with mean root lengths of 11.8 and 14.0 mm,
respectively. High survival percentage of 80% was recorded
for those plantlets raised in “jiffy-7” but survival reduced
to 40% when grown in the autoclaved soil after one week.
Key
words:
Teak (Tectona grandis) plantlets, indole-3-butyric
acid, light regime, in vitro rooting,
acclimatization. |
