Full Length Research Paper

Enterocin from Enterococcus faecium isolated from mangrove environment

N. Annamalai*, P. Manivasagan, T. Balasubramanian and S. Vijayalakshmi

CAS in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.

*Corresponding author. E-mail: annabact@gmail.com.

Accepted 31 October, 2008

   Abstract

Enterococcus faecium isolated from mangrove environment produced enterocin and it showed broad inhibitory spectrum against gram positive and gram negative bacteria such as Lactobacillus plantarum, Enterococcus facealis, Listeria monocytogens and Salmonella paratyphii. The optimum production of bacteriocin (2400 AU/ml) from E. faceium was obtained when the culture conditions were maintained at pH 6.0 and 35°C. Maximum yield was 40% in ion exchange chromatography. The molecular weight of the partially purified enterocin was estimated as 5 KDa by SDS PAGE electrophoresis.

Key words: Enterococcus faecium, Enterocin, antimicrobial spectrum and mangrove environment.