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Effect of plant
growth regulators on in vitro shoot
multiplication of Amygdalus communis L. cv. Yaltsinki
Filiz Akbaş1*, Çiğdem Işıkalan2,
Süreyya Namlı2 and Bekir Erol Ak3
1Department
of Biology, Art and Science Faculty, Batman University,
Batman, Turkey.
2Department
of Biology, Art and Science Faculty, Dicle University,
Diyarbakır, Turkey.
3Department
of Faculty of Agriculture, The University of Harran, Urfa-
Turkey.
*Corresponding author. E-mail:
filiz.akbas@batman.edu.tr
or
filizakbas71@gmail.com. Tel.: +90-488-2157202. Fax:
+90-488-2157201.
Abbreviations:
BA, Benzylaminopurine (N6-benzyladenine);
kin, kinetin; IBA, indole butyric acid;
NAA,
α-naphthaleneacetic acid; IAA, Indole-3-acetic acid;
MS, Murashige and Skoog medium.
Accepted
21 July, 2009 |
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An efficient protocol was established for in vitro
shoot multiplication from apical
shoot tips derived
from mature trees of almond (Amygdalus communis L.)
cultivars, Yaltsinki. Explants were cultured on Murashige
and Skoog (1962) (MS) medium containing various
concentrations of 6-benzyladenin (BA) and kinetin (kin) for
shoot multiplication. Shoot multiplication was best achieved
from explant on MS medium containing 30 gl-1
sucrose, 7 gl-1 agar and 1.0 mgl-1 BA.
This amount of BA (1.0 mgl-1) gave the best
multiple shoot formation response with an average of 16.10
shoots per explant. In addition, shoots were cultured on the
media containing 1.0 mgl-1 BA and kin
combined with three different auxins (0.25 and 0.5 mgl-1
of IAA, IBA and NAA) separately. It was noted that 1.0 mgl-1
BA and kin combinated with NAA had inhibitory effect
on new shoot formation and no shoot formation was induced.
However, explants cultivated on medium containing 1.0 mgl-1
BA and 0.5 mgl-1 IAA resulted in 11.25 shoots per
explant. The effect of four
different sucrose concentrations (20, 30, 40, 50 gl-1)
on the multiplication of shoots was also investigated. The
best shoot multiplication was obtained in MS media
containing 30 gl-1 sucrose with an average of
15.40 shoots per explant.
Key
words:
Amygdalus communis L.cv. Yaltsinki, PGRs, in vitro,
shoot multiplication. |
