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RAPD analysis reveals
genetic variation in different populations of Trichodesma
indicum - A perennial medicinal herb
Neelambra Verma*, V. Koche, K. L. Tiwari and S. K. Mishra
School of
Life Sciences, Pt. Ravishankar Shukla University, Raipur, C.
G. India.
*Corresponding author. E-mail:
neelambara_verma@yahoo.com.
Tel.:
91-.9981513755.
Accepted 29
June, 2009 |
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Random amplified polymorphic DNA (RAPD) markers were used to
assess genetic variation in different populations of
Trichodesma indicum (family - Boraginaceae), a useful
medicinal herb. 50 plants were collected from 4 different
locations of Chhattisgarh, one location of Madhya Pradesh
and in vitro regenerated plants. Genetic variation
was detected among the populations and within each
population including micropropagated plants regenerated from
zygotic embryos. 20 primers generated a total of 121
polymorphic bands out of 125 total bands (96.8%
polymorphism), with an average of 6.05 amplified bands per
primer. The results reveal very high level of genetic
diversity in the species.
Key
words:
Boraginaceae, polymorphism, in vitro, RAPD,
amplification. |
