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In vitro
direct regeneration in mint
from different explants on half strength MS medium
Sadia Sarwar1,
Muhammad Zia1*, Riaz-ur-Rehman1,
Zarrin Fatima1, Riaz Ahmad Sial2 and
Muhammad Fayyaz Chaudhary1
1Plant
Cell and Tissue Culture Lab, Department of Biotechnology,
Quaid-i-Azam University, Islamabad, Pakistan 45320.
2Agriculture
Biotechnology Research Institute, AARI Faisalabad, Pakistan.
*Corresponding author. E-mail:
ziachaudhary@gmail.com. Tel: +92-51-9064-3011.
Abbreviations: MS,
Murashige and Skoog; BAP, N6-benzylaminopurine;
Kin, kinetin; IAA, indole acetic acid; IBA,
indole butyric acid; NAA, naphthaleneacetic acid.
Accepted 30 April, 2009 |
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In vitro
shoot regeneration of Mentha piperita L. was
investigated using shoot meristems, node, internode and
petiole explants. Shoot regeneration was achieved on ½
strength MS salts and vitamins supplemented with various
concentrations of BAP, Kin alone or with NAA. Shoot
meristems and nodal segments showed variation in the
frequency of shoot regeneration with single to multiple
shoots. However, internode and petiole explants were not
potent and failed to regenerate shoots at some of the tested
plant growth regulators concentrations. Variable frequency
of rooting was recorded at four different concentrations of
auxins. The rooted plants were successfully acclimatized to
in vivo conditions in the greenhouse.
Key
words:
Mentha piperita, plant hormone, organogenesis,
shooting. |
