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In vitro
anthelmintic effect of two medicinal plants (Anogeissus
leiocarpus and Daniellia oliveri) on
Haemonchus contortus, an abosomal nematode of
sheep in Burkina Faso
Kabore Adama1*, Belem A. M.
Gaston2, Tamboura Hamidou H.1, Traore
Amadou1 and Sawadogo Laya3
1Institut
de l’Environnement et de Recherches Agricoles (INERA)/Département
Productions Animales (DPA), 04 BP 8645 Ouagadougou/Burkina
Faso.
2Institut
du Développement Rural (IDR)/Université Polytechnique de
Bobo-Dioulasso, 01 BP 3770 Ouagadougou 01, Burkina Faso.
3Université
de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina
Faso.
*Corresponding author.
E-mail: ade1_bf@yahoo.fr.
Tél: (226) 70 28 10 98.
Accepted
12 May, 2009 |
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A study
was conducted to evaluate Anogeissus leiocarpus leaf
and Daniellia oliveri stem barks as effective remedy
for gastrointestinal parasites. The anthelmintic activity of
these extracts on eggs, first stage larvae and adults of
Haemonchus contortus was examined by in vitro
tests. The extracts were prepared to obtain six increasing
concentrations. This was done with Phosphate Buffered Saline
(PBS) for egg hatch,
embryonated egg
assays
(75, 150, 300, 600, 1 200 and 2 400 µg/ml) and adult
inhibition of motility assay (0.25, 0.5, 1, 2, 4 and 8
mg/ml). PBS and levamisole (at 0.125 µg/ml in PBS) were used
as negative and positive control groups, respectively. Both
plant extracts induced anthelmintic effects on the three
life-cycle stages of H. contortus and these effects
were significantly different when they were compared to the
negative control group (PBS) (P<0.05). The effect was
dose-dependent on egg hatching and first stage larvae (L1)
but not on adult worms. Magnitude of effect was proportional
to concentration of plant extracts for egg hatching and L1
but not for adult worms. Besides, the results showed that
the D. oliveri stem bark extract was more ovicidal
and larvicidal than A. leiocarpus leaves. It is
concluded that these two plants do really possess
anthelmintic properties.
Key
words:
Anthelmintic, leaf, stem bark, extracts, Anogeissus
leiocarpus, Daniellia oliveri, Haemonchus
contortus. |
