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Use of AFLPs to
differentiate between Fusarium species causing root
rot disease on durum wheat (Triticum turgidum
L. var. durum)
Mohammad El-Khalifeh, Ahmed El-Ahmed, Ahmad Al-Saleh and
Miloudi Nachit*
International Center for Agricultural Research in the Dry
Areas (ICARDA), P. O. Box 5466, Aleppo, Syria.
*Corresponding author.
E-mail:
m.nachit@cgiar.org.
Tel:
(963-21) 26912402.
Fax: (963-21) 213 490.
Accepted 23
June, 2009 |
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Durum wheat is the main crop in the Mediterranean dryland,
where root rot (Fusarium spp. and Helmintosporium
sativum) diseases are common. The aim of this study was
to use the
AFLP
technique to determine variation and genetic relationships
between Syrian Fusarium isolates; and compare
them with some standard Fusarium species. To identify
the different pathogens causing root rots,
AFLP
templates were prepared by the digestion of
Fusarium DNA with EcoRI and MseI
restriction endonucleases and subsequent ligation
of corresponding site-specific adapters. The
isolates were collected from the main durum wheat growing
provinces in Syria (Aleppo, Hassaka and Daraa). A total of
389 AFLP polymorphic bands were obtained using 3 primer
combinations. AFLP analyses indicated that the isolate
Hassaka31 was the closest to F. poae, Hassaka2 to
F. culmorum. Daraa14, Daraa49 and Tel Hadya16 to
F. equiseti, and Daraa74 and Daraa62 to F.
graminearum. Whereas the isolates Tel Hadya10, Tel
Hadya19 and Aleppo4 were close to F. avenaceum. As
for the cluster analysis based on 41 specific traits
(morphology and microscopy), it showed that Hassaka31 and
Daraa74 were very similar to F. avenaceum. Whereas,
isolates Aleppo4, Daraa14, Daraa49, Tel Hadya16, Daraa62 and
Tel Hadya10 were similar to F. avenaceum,
Hassaka2 to F. culmorum and Tel Hadya19 to
F. equiseti, F. graminearum and F. poae.
Tel Hadya10 and Daraa62 were the most virulent isolates and
they are used in the breeding program for resistance
screening.
Key
words:
DNA, markers, primer, cluster. |
