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Cloning and expression of
a Vi mimotope of Salmonella enterica serovar Typhi
through nucleotide-nucleotide hybridization approach
Kek Heng Chua1,2*,
Boon Pin Kee1 and Kwai Lin Thong3
1Department
of Molecular Medicine, Faculty of Medicine, University of
Malaya, Kuala Lumpur, Malaysia.
2Centre
for Genomics Research, Faculty of Medicine, University of
Malaya, Kuala Lumpur, Malaysia.
3Institute
of Biological Sciences, Faculty of Science, University of
Malaya, Kuala Lumpur, Malaysia.
*Corresponding author. E-mail:
khchua@um.edu.my.
Tel.:603-79676607.
Fax:
603-79676600.
Accepted 10
August, 2009 |
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A recombinant His-Vi protein of Salmonella enterica
serovar Typhi was successfully constructed and cloned into
an expression vector through a nucleotide-nucleotide
hybridization approach. After transformation of the
construct into Escherichia coli, the recombinant
His-Vi protein with a size of approximately 4 kDa was
successfully produced and proven by Western blot analysis.
This recombinant protein can be used to detect specific
anti-Vi antibody produced by typhoid patients. Overall, the
His-Vi recombinant protein could serve as a potential
diagnostic reagent to detect Salmonella Typhi
infection in an individual.
Key
words:
Salmonella enterica serovar Typhi, recombinant
protein, Vi mimotope. |
