Full Length Research Paper

Standardization of RAPD assay for genetic analysis of olive

Meltem Sesli¹* and E. Dilşat Yeğenoğlu²

¹School of Tobacco Expertise, Celal Bayar University, Republic of Turkey, 45210 Akhisar, Manisa, Turkey.

²Akhisar Vocational School, Celal Bayar University, Republic of Turkey, 45210 Akhisar, Manisa, Turkey.

*Corresponding author. E-mail: meltem.sesli@bayar.edu.tr. Tel: (+90) 236 412 68 96. Fax: (+90) 236 413 70 58.

Accepted 6 November, 2009

   Abstract

This study aimed at the standardization of PCR conditions for the purpose of determining the genetic similarities and distances in wild and cultivated olives collected from Izmir and Manisa provinces in Aegean Region. Three different PCR mix and three different PCR cycle as well as 20 primers from OP-I series were tried in 13 wild and 12 cultivated samples. A suitable PCR protocol was developed and evaluable bands were derived for further studies. Required result could not be obtained from PCR Mixes I and II and also PCR cycles I and II; however, favorable results were attained by PCR Mix III and PCR cycle III. Evaluable bands were obtained for defining the olive samples by using primers from OP-I. Thus, it was concluded that RAPD profiles are effective in the study of genetic similarities and distances of wild and cultivated olives.

Key words: Olive, RAPD, PCR, standardization.