|
Microbial conversion of
major ginsenoside Rb1 to minor ginsenoside Rd by Indian
fermented food bacteria
Kalaiselvi Senthil1*,
V. Veena1, M. Mahalakshmi1,
Ramakrishna Pulla2, Deok Chun Yang2
and R. Parvatham1
1Department
of Biochemistry, Biotechnology and Bioinformatics,
Avinashilingam University for Women, Coimbatore-641043,
Tamilnadu, India.
2Department
of Oriental Medicine material and processing, College of
Life Sciences, Kyung Hee University, Suwon, South Korea.
*Corresponding author. E-mail:
kalaisen@yahoo.com.
Tel.: +91- 98-422-42-948.
Accepted
17 April, 2009 |
|
Ginsenoside
Rb1 is the predominant secondary metabolite (saponin) in
Panax ginseng. Hydrolysis of the sugar residues in Rb1
yields more pharmaceutically active ginsenosides like Rd,
Rg3, F2, Rh2 and C-K. Among them, the minor ginsenoside Rd
enhances the differentiation of neural stem cells, protects
neurons from neurotoxic chemicals, decreases urea nitrogen
and creatinine in kidney. It also protects the kidney from
apoptosis and DNA fragmentation caused by cancer and
chemical drugs and is more useful therapeutically than the
major ginsenoside Rb1. Bacteria showing
b-glucosidase
activity were isolated from fermented Indian food using
esculin-MRS agar. Bacteria from Amla in sugar syrup and
Boiled Amla in jaggery syrup converted ginsenoside Rb1 to
minor ginsenoside Rd. TLC and HPLC analysis showed that with
increase in incubation time the conversion of Rb1 to Rd also
increased. The 16s rDNA sequence was determined and the
bacteria showed 93% sequence similarity to Brumimicrobium
mesophilum.
Key
words:
Conversion, ginseng, ginsenoside, Rb1, Rd. |
