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In vitro
mass propagation of Typhonium flagelliforme
as affected by plant growth
regulators
Nobakht, G. M.1, Kadir, M. A.1*
and Stanslas, J.2
1Department of Agriculture Technology, Faculty of Agriculture,
Universiti Putra Malaysia, 43400 Serdang, Selangor Darul
Ehsan, Malaysia.
2Department of Medicine, Faculty of Medicine and Health Science,
Universiti Putra Malaysia, 43400, Serdang, Selangor Darul
Ehsan, Malaysia.
*Corresponding author.
E-mail:
mihdzar@agri.upm.edu.my or
motaharenobakht@yahoo.com.
Tel: +603_8946 4114. Fax:
+603-8946 4146.
Accepted
5 October, 2009 |
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Tubers were used as explants in in vitro
mass propagation of Rodent Tuber (Typhonium flagelliforme).
The explants were obtained from sterile plantlets and placed
in shoot induction medium containing basal salts of
Murashige and Skoog (MS) and various concentrations of
6-benzylaminopurine (BAP) and α-naphthaleneacetic
acid
(NAA).
Treatment containing 5 mg/l (w/v) of BAP with 1 mg/l (w/v)
of NAA produced the highest number of shoots per explant
(29.17) after 12 weeks of culture and also the highest mean
fresh weight of shoots formed in treatment containing 5 mg/l
(w/v) of BAP with 1 mg/l (w/v) of NAA. For ex vitro
establishment, well- rooted plantlets were transferred in
potting medium containing peatmoss, perlite and vermiculite
(3:1:1).
Key
words:
Typhonium, 6-benzylaminopurine, α-naphthaleneacetic
acid, indole-3-butyric acid, in vitro culture, mass
propagation. |
