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Studies on
the proteinaceous gel secretion from the skin of the
catfish, Arius maculatus (Thunberg, 1792)
P. Manivasagan*, N. Annamalai,
S. Ashokkumar and P. Sampathkumar
CAS in Marine Biology,
Annamalai University,
Parangipettai - 608 502, Tamil Nadu, India.
*Corresponding author. E-mail:
manimaribtech@gmail.com.
Accepted 24 October, 2009 |
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The Catfish Arius maculatus (Thunberg, 1792) causes
injury to the fisherman while handling the fish and it was
proven that the skin mucus of the fish have several
properties including the toxicity. In the present study, the
biochemical property of the catfish skin mucus was
characterized and it was found that the protein content of
the soluble and insoluble fractions were 9.34 and 12.64
µg/g, respectively. The total lipid was 0.005 µg/g and the
total carbohydrate was 0.08 µg/g. Of the total 17 amino
acids recorded, cysteine availability was very low; 0.01 and
0.02 mole % in the soluble and insoluble fractions,
respectively. Leucine was available in high quantity (9.8
mole %) in the soluble fraction and aspartic acid (9.0 mole
%) was high in the insoluble fraction. On SDS-PAGE analysis,
seven and six bands with a distinct band at 35 KDa in the
soluble and insoluble fractions, respectively, were
observed. On haemolytic activity, lysis was observed by 50
µg/ml of insoluble fraction and 25 µg/ml of soluble
fraction. Both soluble and insoluble fractions showed
maximum and minimum activities against Escherichia coli
and Pseudomonas aueruginosa, respectively.
Key words:
Catfish, Arius maculates, biochemical
composition, haemolytic activity, antibacterial activity. |
